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QUALITATIVE METHOD
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SEMI QUANTITATIVE METHOD
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1
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Place
one drop of sample/ controls onto separate reaction circles of the
glass slide.
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1
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Using
isotonic saline prepare serial dilutions of the serum sample 1:2,
1:4,1:8,1:16 and so on pipette each serum dilution onto separate
reaction circles of the slide.
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2
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Add
one drop of EUROTEX RF latex reagent to the sample controls on the
slide.
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2
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Add
one drop of EUROTEX RF latex reagent to each dilution of the serum
sample.
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3
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Using
a mixing stick mix the sample and
EUROTEX RF latex reagent uniformly over the entire circle
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3
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Using
a mixing stick mix the sample and the latex reagent uniformly over
the entire circle.
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4
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Immediately
start a stop watch Rock the slide gently back and forth.
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4
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Immediately
start a stopwatch. Rock the slide gently back and forth.
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5
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Observe
for agglutination macroscopically at two minutes.
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5
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Observe
for agglutination macroscopically at two minutes.
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INTERPRETATION
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Agglutination
is a positive test result and indicates the presence of detectable
levels of Rheumatoid Factors
No
Agglutination is a negative test result and indicates the absence of
detectable levels of rheumatoid factors.
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RF
conc. (IU/mL) =12xd
Where
D = Highest dilution of serum showing agglutination.
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